Articles | Volume 8, issue 1
https://doi.org/10.5194/ms-8-23-2017
https://doi.org/10.5194/ms-8-23-2017
Research article
 | 
14 Mar 2017
Research article |  | 14 Mar 2017

Mitochondrial activity detected by cantilever based sensor

Petar Stupar, Wojciech Chomicki, Caroline Maillard, David Mikeladze, Aleksandar Kalauzi, Ksenija Radotić, Giovanni Dietler, and Sandor Kasas

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Cited articles

Aghayee, S., Benadiba, C., Notz, J., Kasas, S., Dietler, G., and Longo, G.: Combination of fluorescence microscopy and nanomotion detection to characterize bacteria, J. Mol. Recognit., 26, 590–595, https://doi.org/10.1002/jmr.2306, 2013.
Alonso-Sarduy, L., De Los Rios, P., Benedetti, F., Vobornik, D., Dietler, G., Kasas, S., and Longo, G.: Real-time monitoring of protein conformational changes using a nano-mechanical sensor, PloS One, 9, e103674, https://doi.org/10.1371/journal.pone.0103674, 2014.
Bowler, M. W., Montgomery, M. G., Leslie, A. G. W., and Walker, J. E.: How azide inhibits ATP hydrolysis by the F-ATPases, P. Natl. Acad. Sci. USA, 103, 8646–8649, https://doi.org/10.1073/pnas.0602915103, 2006.
Hong, S. and Pedersen, P. L.: ATP Synthase and the Actions of Inhibitors Utilized To Study Its Roles in Human Health, Disease, and Other Scientific Areas, Microbiol. Mol. Biol. Rev., 72, 590–641, https://doi.org/10.1128/MMBR.00016-08, 2008.
Kasas, S., Ruggeri, F. S., Benadiba, C., Maillard, C., Stupar, P., Tournu, H., Dietler, G., and Longo, G.: Detecting nanoscale vibrations as signature of life, P. Natl. Acad. Sci. USA, 112, 378–381, https://doi.org/10.1073/pnas.1415348112, 2015.
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Short summary
Mitochondria are small organelles with key functions inside our cells. Unfortunately, there are a number of diseases that arise from their malfunction. To test whether they are healthy or not, complicated procedures are often required. In the paper, we applied our sensor that detects motion of living microorganisms. We saw that a sensor detecting tiny vibrations is powerful enough to sense the difference between normal and inhibited mitochondria.